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Subject: Why COA Net Content Matters Even More With Peptide Blends
From: Derek from Research Radar <derekpruski@substack.com>
To: tjphuhs@gmail.com
Message-Id: <20260414050809.3.3df4dbc351075d45@mg-d0.substack.com>
Date: Tue, 14 Apr 2026 05:08:09 +0000
Feedback-Id: post-194152709:cat-post:pub-3365367:substack
Sender: Derek from Research Radar <derekpruski@substack.com>
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Reply-To: Derek from Research Radar
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View this post on the web at https://derekpruski.substack.com/p/why-coa-net=
-content-matters-even

For research purposes only. Not for human consumption. Not medical advice.
First, what is net content and why does it matter?
When a vendor sends a vial to a third-party lab for testing, the COA will s=
how more than just purity. It also shows how much peptide is actually in th=
e vial =E2=80=94 that=E2=80=99s the net content. This number matters becaus=
e the label isn=E2=80=99t always accurate.
A vial labeled 5 mg might actually contain 4.6 mg or 5.3 mg
That variance is common and not automatically a red flag
But if you don=E2=80=99t check it, your research amounts are based on a num=
ber that may not be real
Single peptide vials are easier to work with here =E2=80=94 one variance, o=
ne adjustment
So what changes with blends?
A simple two-peptide blend like BPC-157 / TB-500 is still manageable. If a =
vial comes back at 10.2 mg and 11.4 mg instead of the labeled 11 mg / 11 mg=
, you can account for that and adjust your research amounts. Not ideal, but=
 workable.
The problem compounds when you get into three and four-peptide blends.
Imagine a PE2228 / Semax / Selank / Pinealon blend intended to be 12 / 12 /=
 12 / 12
If the COA comes back 16 / 11 / 8 / 9, the ratios are completely skewed
Each of those peptides targets a different pathway
You can=E2=80=99t selectively redose one to correct the imbalance =E2=80=94=
 the whole protocol is off
The more peptides in a blend, the more variables a vendor has to control at=
 once, and the more ways it can go wrong
The takeaway
Complex multi-peptide blends are harder to quality control, harder to verif=
y, and harder to course correct when something is off. This is one of the m=
ain reasons I generally steer toward singles or simple two-peptide blends i=
n my own research =E2=80=94 more control, easier to interpret results, easi=
er to troubleshoot.
Always pull the COA before researching any compound
Don=E2=80=99t just check purity =E2=80=94 check net content for every pepti=
de in the blend
If a vendor isn=E2=80=99t testing their blends to the same standard as thei=
r singles, take note
Simpler is almost always better when it comes to blend complexity
All content is intended for research and educational purposes only. Not for=
 human consumption. This is not medical advice. Always conduct your own due=
 diligence.

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@media screen and (max-width: 650px) {
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  body .markup div.captioned-button-wrap .subscription-widget.show-subscrib=
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@media screen and (max-width: 650px) {
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@media (max-width: 650px) {
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  body .markup .tweet {
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}
@media (max-width: 650px) {
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}
@media (max-width: 650px) {
  body .markup .twitter-embed .tweet-photos-container.two,
  body .markup .tweet .tweet-photos-container.two,
  body .markup .twitter-embed .tweet-photos-container.three,
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}
@media (max-width: 650px) {
  body .markup .twitter-embed a.expanded-link .expanded-link-img,
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}
@media (max-width: 650px) {
  body .markup .twitter-embed a.expanded-link .expanded-link-description,
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}
@media screen and (max-width: 650px) {
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}
@media (max-width: 420px) {
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mg {
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}
@media (max-width: 420px) {
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@media (max-width: 420px) {
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@media screen and (min-width: 500px) {
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@media screen and (max-width: 420px) {
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@media screen and (max-width: 480px) {
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@media screen and (max-width: 650px) {
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@media screen and (min-width: 500px) {
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@media screen and (max-width: 650px) {
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@media screen and (max-width: 650px) {
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@media screen and (max-width: 650px) {
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@media (max-width: 650px) {
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@media screen and (max-width: 650px) {
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@media screen and (max-width: 650px) {
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@media screen and (min-width: 541px) {
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@media screen and (max-width: 768px) {
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@media screen and (max-width: 768px) {
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@media screen and (max-width: 768px) {
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@media only screen and (min-width: 630px) {
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@media screen and (min-width: 541px) {
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@media screen and (max-width: 650px) {
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@media screen and (max-width: 650px) {
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@media screen and (max-width: 650px) {
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@media screen and (max-width: 650px) {
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@media (min-width: 250px) {
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@media (min-width: 300px) {
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@media (min-width: 350px) {
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@media (min-width: 350px) {
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@media (min-width: 400px) {
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@media (min-width: 600px) {
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}
@media (max-width: 650px) {
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@media (max-width: 750px) {
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}</style></head><body class=3D"email-body" style=3D"font-kerning: auto;--im=
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ne;font-size:1px;color:#333333;line-height:1px;max-height:0px;max-width:0px=
;opacity:0;overflow:hidden;">For research purposes only.</div><div class=3D=
"preview" style=3D"display:none;font-size:1px;color:#333333;line-height:1px=
;max-height:0px;max-width:0px;opacity:0;overflow:hidden;">&#847; &nbsp; &#8=
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#173;&#847; &nbsp; &#8199; &#173;</div><table class=3D"email-body-container=
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td></td></tr><tr><td></td><td class=3D"content" width=3D"550" align=3D"left=
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00%;margin: 0 auto;overflow-wrap: break-word;"><table role=3D"presentation"=
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d style=3D"vertical-align:middle;"><span class=3D"pencraft pc-reset reset-I=
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</tbody></table></td></tr><tr height=3D"16"><td height=3D"16" style=3D"font=
-size:0px;line-height:0;">&nbsp;</td></tr></tbody></table></div></div><div =
class=3D"post typography" dir=3D"auto" style=3D"--image-offset-margin: -120=
px;padding: 32px 0 0 0;font-size: 16px;line-height: 26px;"><div class=3D"bo=
dy markup" dir=3D"auto" style=3D"text-align: initial;font-size: 16px;line-h=
eight: 26px;width: 100%;word-break: break-word;margin-bottom: 16px;"><p sty=
le=3D"margin: 0 0 20px 0;color: rgb(54,55,55);line-height: 26px;font-size: =
16px;margin-top: 0;"><em>For research purposes only. Not for human consumpt=
ion. Not medical advice.</em></p><div style=3D"font-size: 16px;line-height:=
 26px;"><hr style=3D"margin: 32px 0;padding: 0;height: 1px;background: rgb(=
0,0,0,.1);border: none;"></div><p style=3D"margin: 0 0 20px 0;color: rgb(54=
,55,55);line-height: 26px;font-size: 16px;"><strong>First, what is net cont=
ent and why does it matter?</strong></p><p style=3D"margin: 0 0 20px 0;colo=
r: rgb(54,55,55);line-height: 26px;font-size: 16px;">When a vendor sends a =
vial to a third-party lab for testing, the COA will show more than just pur=
ity. It also shows how much peptide is actually in the vial &#8212; that&#8=
217;s the net content. This number matters because the label isn&#8217;t al=
ways accurate.</p><ul style=3D"margin-top: 0;padding: 0;"><li style=3D"marg=
in: 8px 0 0 32px;mso-special-format: bullet;"><p style=3D"color: rgb(54,55,=
55);line-height: 26px;margin-bottom: 0;box-sizing: border-box;padding-left:=
 4px;font-size: 16px;margin: 0;">A vial labeled 5 mg might actually contain=
 4.6 mg or 5.3 mg</p></li><li style=3D"margin: 8px 0 0 32px;mso-special-for=
mat: bullet;"><p style=3D"color: rgb(54,55,55);line-height: 26px;margin-bot=
tom: 0;box-sizing: border-box;padding-left: 4px;font-size: 16px;margin: 0;"=
>That variance is common and not automatically a red flag</p></li><li style=
=3D"margin: 8px 0 0 32px;mso-special-format: bullet;"><p style=3D"color: rg=
b(54,55,55);line-height: 26px;margin-bottom: 0;box-sizing: border-box;paddi=
ng-left: 4px;font-size: 16px;margin: 0;">But if you don&#8217;t check it, y=
our research amounts are based on a number that may not be real</p></li><li=
 style=3D"margin: 8px 0 0 32px;mso-special-format: bullet;"><p style=3D"col=
or: rgb(54,55,55);line-height: 26px;margin-bottom: 0;box-sizing: border-box=
;padding-left: 4px;font-size: 16px;margin: 0;">Single peptide vials are eas=
ier to work with here &#8212; one variance, one adjustment</p></li></ul><p =
style=3D"margin: 0 0 20px 0;color: rgb(54,55,55);line-height: 26px;font-siz=
e: 16px;"><strong>So what changes with blends?</strong></p><p style=3D"marg=
in: 0 0 20px 0;color: rgb(54,55,55);line-height: 26px;font-size: 16px;">A s=
imple two-peptide blend like BPC-157 / TB-500 is still manageable. If a via=
l comes back at 10.2 mg and 11.4 mg instead of the labeled 11 mg / 11 mg, y=
ou can account for that and adjust your research amounts. Not ideal, but wo=
rkable.</p><p style=3D"margin: 0 0 20px 0;color: rgb(54,55,55);line-height:=
 26px;font-size: 16px;">The problem compounds when you get into three and f=
our-peptide blends.</p><ul style=3D"margin-top: 0;padding: 0;"><li style=3D=
"margin: 8px 0 0 32px;mso-special-format: bullet;"><p style=3D"color: rgb(5=
4,55,55);line-height: 26px;margin-bottom: 0;box-sizing: border-box;padding-=
left: 4px;font-size: 16px;margin: 0;">Imagine a PE2228 / Semax / Selank / P=
inealon blend intended to be 12 / 12 / 12 / 12</p></li><li style=3D"margin:=
 8px 0 0 32px;mso-special-format: bullet;"><p style=3D"color: rgb(54,55,55)=
;line-height: 26px;margin-bottom: 0;box-sizing: border-box;padding-left: 4p=
x;font-size: 16px;margin: 0;">If the COA comes back 16 / 11 / 8 / 9, the ra=
tios are completely skewed</p></li><li style=3D"margin: 8px 0 0 32px;mso-sp=
ecial-format: bullet;"><p style=3D"color: rgb(54,55,55);line-height: 26px;m=
argin-bottom: 0;box-sizing: border-box;padding-left: 4px;font-size: 16px;ma=
rgin: 0;">Each of those peptides targets a different pathway</p></li><li st=
yle=3D"margin: 8px 0 0 32px;mso-special-format: bullet;"><p style=3D"color:=
 rgb(54,55,55);line-height: 26px;margin-bottom: 0;box-sizing: border-box;pa=
dding-left: 4px;font-size: 16px;margin: 0;">You can&#8217;t selectively red=
ose one to correct the imbalance &#8212; the whole protocol is off</p></li>=
<li style=3D"margin: 8px 0 0 32px;mso-special-format: bullet;"><p style=3D"=
color: rgb(54,55,55);line-height: 26px;margin-bottom: 0;box-sizing: border-=
box;padding-left: 4px;font-size: 16px;margin: 0;">The more peptides in a bl=
end, the more variables a vendor has to control at once, and the more ways =
it can go wrong</p></li></ul><p style=3D"margin: 0 0 20px 0;color: rgb(54,5=
5,55);line-height: 26px;font-size: 16px;"><strong>The takeaway</strong></p>=
<p style=3D"margin: 0 0 20px 0;color: rgb(54,55,55);line-height: 26px;font-=
size: 16px;">Complex multi-peptide blends are harder to quality control, ha=
rder to verify, and harder to course correct when something is off. This is=
 one of the main reasons I generally steer toward singles or simple two-pep=
tide blends in my own research &#8212; more control, easier to interpret re=
sults, easier to troubleshoot.</p><ul style=3D"margin-top: 0;padding: 0;"><=
li style=3D"margin: 8px 0 0 32px;mso-special-format: bullet;"><p style=3D"c=
olor: rgb(54,55,55);line-height: 26px;margin-bottom: 0;box-sizing: border-b=
ox;padding-left: 4px;font-size: 16px;margin: 0;">Always pull the COA before=
 researching any compound</p></li><li style=3D"margin: 8px 0 0 32px;mso-spe=
cial-format: bullet;"><p style=3D"color: rgb(54,55,55);line-height: 26px;ma=
rgin-bottom: 0;box-sizing: border-box;padding-left: 4px;font-size: 16px;mar=
gin: 0;">Don&#8217;t just check purity &#8212; check net content for every =
peptide in the blend</p></li><li style=3D"margin: 8px 0 0 32px;mso-special-=
format: bullet;"><p style=3D"color: rgb(54,55,55);line-height: 26px;margin-=
bottom: 0;box-sizing: border-box;padding-left: 4px;font-size: 16px;margin: =
0;">If a vendor isn&#8217;t testing their blends to the same standard as th=
eir singles, take note</p></li><li style=3D"margin: 8px 0 0 32px;mso-specia=
l-format: bullet;"><p style=3D"color: rgb(54,55,55);line-height: 26px;margi=
n-bottom: 0;box-sizing: border-box;padding-left: 4px;font-size: 16px;margin=
: 0;">Simpler is almost always better when it comes to blend complexity</p>=
</li></ul><p style=3D"margin: 0 0 20px 0;color: rgb(54,55,55);line-height: =
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